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Tuesday, June 22, 2010
read a book called 小女人nice. so sad that i've finished it. now i can either start revision proper, or read another book. (after spending the entire morning slacking on the internet) finished the book last night. sian, dont want to revise since i'm never gonna catch up anyway-.- not at this late hour, nope. spent a whooping entire day in school yesterday, doing science society project. we're investigating the effect of chromosome 4 on longevity. shiyi's overseas, so sandy and i let Dr Yap help us subculture some T-cells, then we went on to do PCR-ing and electrophoresis. we divided our stock cells into 2 portions, then used 1 of it for PCR-ing. Added MgCl2, dNTP, forward and backward primers, Taq, and nuclease-free water into a really small tube, and after multiple times of centrifuginging, we finally got the tube into the PCR machine. Then we made the electrophoresis gel using agarose powder, some biosafe stain, deionised water, and TBE buffer solution. let it set, and finally went for a late lunch. hungryy. we came back, and continued waiting for the PCR to run. almost 3 hours later, we finally got it ready, only to realise that we had forgotten to add in the lysis buffer and the RNAase so the entire day was essentially wasted. ): ): ): Ms Lee said "aiyo liddat then your common test cannot make it le" when she asked me how revision was coming along and i said i havent started. Sandy started asking Ms Lee chem questions while i tried to remember what Kc was. sigh. i honestly though our project was heading somewhere. now Dr Yap'll get us to change project cos we've been procrastinating and wont have enough time to continue with this particular one. ): piano, sc meeting for teachers' day, then yog pottery training coming up. i dont remember when Ms Chew's outing is. ): __________________________________________________________ edit, i forgot to mention 4K's night event. it was :D, albeit small. watched YES MAN, some jackie chan spy movie, shutter island. ive watched shutter island before (on the flight to europe), so it wasnt much of a loss when i went off towards the end to get some sleep (had to reach school the subsequent morning for science society at 9am!). had dinner at T1, and heard a group of ladies singing happy birthday in korean. wanz wanted to play chubby bunny with marshmallows, but she left before we did. so we didnt. jo left at 12am, when her fairymother called her(: tzyy, huang, sam, char left with wan. conclusion: great dinner, great movies, great company. yup. its 1.39pm. goodbye. |
Drakon
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